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Raw 264.7 cells and human HepG2 cells were cultured in DMEM supplemented with 10% v / v fetal bovine serum (FBS), penicillin (100 U / mL, streptomycin (100 g / while maintaining a 5% CO 2 and incubated. before carrying out the experiment hepatocellular toxicity induced by the LPS was confirmed that the toxicity of the extract. a 96 well plate divided by the cell density 2.5x10 5 cells / mL in cell adhesion for the next 24 hours After the incubation, the samples were treated at different concentrations to determine the cytotoxicity of the cells.
Referring to FIG. 5, HepG2 cells were plated in a 24-well plate at 5 × 10 5 cells / well, and then incubated for 18 hours in DMEM without FBS.
THP-1 cells associated with the inflammatory reaction were suspended in RPMI 1640 medium containing 10% FBS to a concentration of 2.0 x 10 4 cells / well, and 200 μL of each was inoculated into each well of a 96-well plate.
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